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t4 ligase buffer (New England Biolabs)

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New England Biolabs t4 ligase buffer
T4 Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2513 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t4+dna+ligase+reaction+buffer/T4+DNA+Ligase+Reaction+Buffer/pm42883912-218-32-23
Average 99 stars, based on 2513 article reviews

t4 ligase buffer - by Bioz Stars, 2026-10

99/100 stars

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Phospho-proteomics:

Article Title: Reprogramming of neuronal genome function and phenotype by astrocytes
Article Snippet: Forward and reverse oligonucleotides representing the CRISPR sgRNA protospacer sequence were designed and synthesized (Integrated DNA Technologies or Genewiz) with 5’-CACCG-forward oligonucleotides-3’ and 5’-AAAC-reverse oligonucleotides-C-3’ architectures, respectively. .. Phosphorylation and annealing were performed by mixing 500 ng of each strand with T4 Polynucleotide Kinase (NEB, M0201) in T4 DNA Ligase Reaction Buffer (NEB, M0202), incubating at 37°C for 30 minutes, 95°C for 5 minutes, and ramping down to 25°C at 5°C/min. .. The annealed duplex was diluted 1:200 and cloned into Esp3I-digested (NEB, R0734) and gel-purified (Qiagen, 28706) lentiviral backbone (described above) via T4 DNA ligation (NEB, M0202).

other:

Article Title: LSD1 inhibitor, TAS1440, disrupts INSM1-LSD1 complex activating tumor-suppressive pathways via transcriptional reprogramming in neuroendocrine SCLC
Article Snippet: Assays for histone lysine demethylases (JARID1A, JARID1B, UTX, JMJD2B, and JMJD3), histone lysine methyltransferases (SMYD2 and EZH1/EED/SUZ12), histone deacetylase (HDAC1), DNA methyltransferase (DNMT1), and protein arginine N‐methyltransferase (PRMT4) were performed by Eurofins Cerep SA (Celle‐Lévescault, France).

Article Title: Landscape-scale navigation unlocks antibody CDR structural logic for AI-guided rescue and therapeutic optimization
Article Snippet: The integrity of the annealed dsDNA fragments was confirmed by 18% PAGE gel and detected after SYBR Gold staining (S11494; Thermo Fisher Scientific).

Polymerase Chain Reaction:

Article Title: Dual-site phosphorylation of SLX4 stabilizes the SLX4-MUS81 interface to promote mitotic SMX assembly and genome protection.
Article Snippet: The PCR products were ligated with 400 U T4 DNA Ligase (NEB) in 1 × T4 DNA Ligase Reaction Buffer (NEB) overnight at 16 ◦C. .. The PCR products were ligated with 400 U T4 DNA Ligase (NEB) in 1 × T4 DNA Ligase Reaction Buffer (NEB) overnight at 16 ◦C. ..